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Anti-KDM6B / JMJD3 antibody
Anti-KDM6B / JMJD3 antibody from Abcam
Be the first to write a review!
Citations:
(45)
Supplier Page
Supplier Page from
Abcam for
Anti-KDM6B / JMJD3 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-KDM6B / JMJD3 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab38113
Clonality
Polyclonal
Quantity
100 UG
Conjugate/Tag
Unconjugated
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Concentration
1 mg/ml
Research Area
Epigenetics
Gene Name
KDM6B
Target
KDM6B / JMJD3
Reactivity
Human (Hu)
Host
Rabbit
Isotype
IgG
Antibody Type
Primary
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (45)
(1)
KDM6B drives epigenetic reprogramming associated with lymphoid stromal cell early commitment and immune properties
Science Advances
December 1, 2023
Marvin Sylvestre, Nicolas Barbier, Vonick Sibut, Saba Nayar, Céline Monvoisin, Simon Leonard, Julien Saint-Vanne, Ansie Martin, Marion Guirriec, Maëlle Latour,
et al.
C15410046
, polyclonal) antibodies for 1 hour at room temperature, or with 1 μl of anti-KDM6B (
Abcam
,
ab38113
, RRID:AB_943898) or 2 μl of
p300
(
Diagenode
,
C15200211
) antibodies, overnight at 4°C. For each experiment,
(2)
KDM6B promotes gastric carcinogenesis and metastasis via upregulation of CXCR4 expression
Cell death & disease
December 23, 2022
Liu F, Wang Y, Yang Z, Cui X, Zheng L, Fu Y, Shao W, Zhang L, Yang Q, Jia J.
samples, and then analyzed using
qPCR
. The antibodies used in this study are as follows: anti-
KDM6B
(
ab38113
,
Abcam
, Cambridge, UK), anti-H3K27me3 (
ab6002
,
Abcam
), and anti-IgG (
2729
,
Cell Signaling Technology
).
(3)
JMJD3 suppresses tumor progression in oral tongue squamous cell carcinoma patients receiving surgical resection
PeerJ
July 13, 2022
Yen-Hao Chen, Chang-Han Chen, Chih-Yen Chien, Yan-Ye Su, Sheng-Dean Luo, Shau-Hsuan Li
peroxide for 5 min. After that, the specimen was reacted with primary antibody against
JMJD3
(
Abcam
,
ab38113
,
1:100
, Cambridge, UK), and then with a ready-to-use visualization reagent consisting of
goat
secondary
(4)
JMJD3 intrinsically disordered region links the 3D-genome structure to TGFβ-dependent transcription activation
Nature communications
June 7, 2022
Vicioso-Mantis M, Fueyo R, Navarro C, Cruz-Molina S, van Ijcken WFJ, Rebollo E, Rada-Iglesias Á, Martínez-Balbás MA.
acids 798–1095, dilution
1:200
for
immunofluorescence (IF
),
1:1000
for western blot
(WB); and
Abcam
,
ab38113
, dilution
1:250
for IF), DAPI (
ThermoFisher
,
D1306
, dilution
1:500
for IF), β-TUBULIN (
Millipore
,
MAB3408
,
(5)
Downregulation of the endothelial histone demethylase JMJD3 is associated with neointimal hyperplasia of arteriovenous fistulas in kidney failure
The Journal of biological chemistry
May 1, 2022
Feng S, Peden EK, Guo Q, Lee TH, Li Q, Yuan Y, Chen C, Huang F, Cheng J.
(
SouthernBiotech
; catalog no.:
0100-20
) was used to stain nuclear DNA. Rabbit anti-JMJD3 (
Abcam
; catalog no.:
ab38113
), rabbit anti-H3K27me3 antibody (
Abclonal
; catalog no.:
A2363
), rabbit anti-α-SMA antibody (
Sigma
; catalog
(6)
Identification and Characterization of a Novel Long Noncoding RNA that Regulates Osteogenesis in Diet-Induced Obesity Mice
Frontiers in Cell and Developmental Biology
April 21, 2022
Zhekai Hu et al.
DNA was sheared to 200–1,000 base pairs for further
immunoprecipitation
with KDM6B (
1:50
, Cat. No.
ab38113
,
Abcam
, MA, United States) and H3K27me3 (
1:50
, Cat. No.
9733
,
Cell Signaling Technology
, MA, United
(7)
Exosomal microRNA-22-3p alleviates cerebral ischemic injury by modulating KDM6B/BMP2/BMF axis
Stem Cell Research & Therapy
December 1, 2021
Yamei Zhang, Junying Liu, Mi Su, Xin Wang, Chenchen Xie
A/G-beads was added to remove
chromatin
components. Then, anti-H3K27me3 antibody and anti-KDM6B antibody (
ab38113
,
ABCAM
) was added for
incubation
, with anti-rabbit IgG (
ab171870
,
ABCAM
), or anti-mouse IgG (
ab81032
,
(8)
SMARCA4 deficient tumours are vulnerable to KDM6A/UTX and KDM6B/JMJD3 blockade
Nature Communications
December 1, 2021
Octavio A. Romero et al.
Signaling); anti-KDM6B (JMJD3) #
3457
(
1:1000
,
Cell Signaling
) for
western blots
or
anti-KDM6B (JMJD3
)
ab38113
(
Abcam
) for immunostaining (see also Supplementary Table 1). For
western blots
, whole-cell lysates were
(9)
KDM6B promotes ESCC cell proliferation and metastasis by facilitating C/EBPβ transcription
BMC Cancer
December 1, 2021
Mei Qin et al.
summarized in Table 1. Tissue sections were incubated with rabbit anti-
human
KDM6B antibody (1100,
ab38113
,
Abcam
, Cambridge, UK),
IHC
scoring was blindly assessed by two Pathology doctor. The
immunohistochemistry
(10)
Activation of epigenetic regulator KDM6B by
Salmonella
Typhimurium enables chronic infections
Gut Microbes
October 26, 2021
Sarika Rana, Sonalika Maurya, Gayatree Mohapatra, Savita Singh, Rohan Babar, Hridya Chandrasekhar, Garima Chamoli, Deepak Rathore, Pallavi Kshetrapal, C. V. Srikanth
transferred to nitrocellulose
membrane
(BioRad). Blots were probed with antibodies against KDM6B (
Abcam
-
ab38113
), Actin (
CST
-
4970S
), Tubulin (
Sigma
-
T7816
), PPARδ (
Abcam
-
ab8937
),
Ezh2
(
CST
-
5246S
). In order to analyze
(11)
YAP1 promotes high glucose‑induced inflammation and extracellular matrix deposition in glomerular mesangial cells by modulating NF‑κB/JMJD3 pathway
Experimental and Therapeutic Medicine
September 23, 2021
Yan Wang, Jinmei Xu, Zhifeng Cheng
1:1,000
), p-65 (cat. no. ab32536; dilution,
1:1,000
), Jumonji domain-containing protein D3 (JMJD3; cat. no.
ab38113
; dilution,
1:1,000
) and GAPDH (cat. no.
ab9485
; dilution,
1:1,000
) overnight at 4˚C (all from
Abcam
).
(12)
Histone demethylase KDM6B inhibits breast cancer metastasis by regulating Wnt/β‐catenin signaling
FEBS Open Bio
August 1, 2021
Jing Xun, Ruifang Gao, Botao Wang, Yifan Li, Yuan Ma, Jun Guan, Qi Zhang
the
membranes
were incubated overnight at 4 °C with the appropriate antibodies, including anti‐KDM6B (
ab38113
;
Abcam
, Cambridge, MA, USA), β‐catenin (
93790
;
Cell Signaling Technology
, Danvers, MA, USA) and β‐actin
(13)
Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer
Nature communications
July 21, 2021
Wang W, Tang YA, Xiao Q, Lee WC, Cheng B, Niu Z, Oguz G, Feng M, Lee PL, Li B,
et al
.
RRID:AB_2277463),
HDAC1
(Upstate, Cat#05-614),
HDAC2
(Upstate, Cat#05-814, RRID:AB_310022), JMJD3 (
Abcam
, Cat#
ab38113
, RRID:AB_943898),
EZH2
(
Cell Signaling
, Cat#
5246
, RRID:AB_10694683), H3K9Ac (Upstate, Cat#07-352, RRID:AB_310544),
(14)
RETRACTED: H3K27 demethylase KDM6B aggravates ischemic brain injury through demethylation of IRF4 and Notch2-dependent SOX9 activation
Molecular Therapy - Nucleic Acids
June 4, 2021
Lisha Chang, Zhaowang An, Jiang Zhang, Fuling Zhou, Dali Wang, Jian Liu, Yunhe Zhang
room temperature for 20 min, followed by the addition of primary rabbit anti-
human
KDM6B
(5 μg/mL,
ab38113
;
Abcam
, Cambridge, UK) dropwise, incubated overnight at 4°C, and washed three times with 0.1M PBS (5
(15)
Cancer-derived exosomal miR-138-5p modulates polarization of tumor-associated macrophages through inhibition of KDM6B
Theranostics
May 3, 2021
Jing Xun et al.
analysis was performed according to published protocols. Primary antibodies were as follows: KDM6B (
ab38113
,
Abcam
, Inc. Cambridge, MA), CD63 (ab193349), Alix (
sc-99010
,
Santa Cruz Biotechnology, Inc
., Santa
(16)
Unlocking the Recovery Potential: JMJD3 Inhibition-Mediated SAPK/JNK Signaling Inactivation Supports Endogenous Oligodendrocyte-Lineage Commitment Post Mammalian Spinal Cord Injury
Neurochemical Research
April 1, 2021
Zhang Bo-Yin, Zhu Qingsan, Ma Yihang, Yang Fan, Zhu Yuhang, Chang Pengyu
Laboratory (Beijing, China). Reagents and Antibodies The
JMJD3
antibodies were from
CST
(#
3457
) and
Abcam
(
ab38113
). The #3457 was for
immunoblot
assay
and
ab38113
was
for IHC
staining
. The
Olig2
antibody was from
Abcam
(
ab109186
).
(17)
The miRNA‐15b/USP7/KDM6B axis engages in the initiation of osteoporosis by modulating osteoblast differentiation and autophagy
Journal of Cellular and Molecular Medicine
February 1, 2021
Xiaohui Lu, Yuantao Zhang, Yin Zheng, Bin Chen
UK), myc (
ab32072
,
1:100
,
Abcam
, Cambridge, UK),
USP7
(
ab109109
,
1:1000
,
Abcam
, Cambridge, UK), KDM6B (
ab38113
,
1:100
,
Abcam
, Cambridge, UK) or anti‐FLAG antibody (
ab205606
,
1:1000
,
Abcam
, Cambridge, UK) and 15
(18)
KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer
Cell death & disease
January 6, 2021
Cao Z, Shi X, Tian F, Fang Y, Wu JB, Mrdenovic S, Nian X, Ji J, Xu H, Kong C,
et al
.
sc-398844
).
Immunohistochemistry
Immunohistochemistry
was conducted to assess the
protein expression
of KDM6B (
Abcam
,
ab38113
), ARs (
Cell Signaling Technology
,
5153
), and CCND1 (Abgent,
AP2612d
). The expression levels of KDM6B,
(19)
Colonic vitamin D receptor expression is inversely associated with disease activity and jumonji domain-containing 3 in active ulcerative colitis
World Journal of Gastroenterology
December 14, 2020
Hong-Qian Wang, Wen-Hui Zhang, Ya-Qi Wang, Xiao-Pan Geng, Ming-Wei Wang, Yuan-Yuan Fan, Jing Guan, Ji-Long Shen, Xi Chen
(
Ab3508
; dilution
1:2000
;
Abcam
Inc., Cambridge, United Kingdom), anti-Jmjd3 rabbit monoclonal antibody (
Ab38113
;
1:400
Abcam
Inc.) or anti-H3K27me3
mouse
monoclonal antibody (
Ab6002
;
1:200
;
Abcam
Inc.) were added
(20)
Jmjd3 Plays Pivotal Roles in the Proper Development of Early-Born Retinal Lineages: Amacrine, Horizontal, and Retinal Ganglion Cells
Investigative ophthalmology & visual science
September 1, 2020
Iwagawa T, Honda H, Watanabe S.
cryostat (CM3050S, Leica). Primary antibodies used are followed; rabbit immunoglobulin G (IgG) anti-JMJD3 (
ab38113
;
Abcam
, Cambridge, MA, USA), -active caspase-3 (G748A; Promega, Madison, WI, USA), -
RBPMS
(
ab194213
;
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Anti-KDM6B / JMJD3 antibody from Abcam
Supplier Page
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Pricing Info
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Company Info
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